Mansoureh Nazari Vishkaeia
Universiti Sains Malaysia, Malaysia
Title: Anti-angiogenic Efficacy Enhancement in Nano Phospholipid complex of Orthosiphon Stamineus Ethanolic Extract
Biography
Biography: Mansoureh Nazari Vishkaeia
Abstract
Orthosiphon stamineus (O. stamineus) is a therapeutic herb with significant pharmacological properties. High dose of O.S extract had demonstrated some potent antiangiogenic and antitumor activities. It had weak competency to cross the biological membranes because of consisting of large size of rings in its active compounds and highly water solubility. This study aims to develop and characterize new O.S-phospholipid complex to reveal more antiangiogenic efficacy by incorporating with phosphatidylcholine to form new cell like structure which is amphipathic in nature.
O.S-Phospholipid complex was prepared by the modified film method, and characterized for its physico-chemical properties. Ex-vivo and in-vitro antiangiogenic studies demonstrated efficacy improvement after formulation.
Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), particle size and HPLC analysis confirm the formation of phospholipid complex. Phospholipid complex of O.S solubility increased in active compounds with 89.21±45.44% entrapment. Ex-vivo study showed 50% improvement in antiangiogenic activity at high dose. Inter (n=3) and intra (n=5) lab experiment were used for confirmation.
Thus, this study demonstrated new nano O.S-Phospholipid complex as a promising method to enhance antiangiogenic activity. Moreover, this method can be used as a sustained delivery system for hydrophilic compounds with poor lipid-solubility and low oral bioavailability which improves pharmacological properties.