Seongjae Jo studied control and measurement theory at the Department of Control and Instrumentation Engineering at Korea University. Among various subjects, He was interested in sensors and went to graduate school to study nano and biosensors. He is currently studying for the 5th semester of graduate school as a master and doctor Integrated course. He is studying sensing methods using optics such as absorbance, Raman scattering, and fluorescence, and he is also studying the synthesis of gold nanoparticles for optical applications. His study is supported by the National Research Foundation of Korea (NRF) under Grant no. NRF-2015R1A1A1A05027581 and Korea University Future Research Grant.
As the modern technology has developed, the problem to toxicity of nano-scale materials continues to rise, so it has emerged as an important research project to detect the toxic agents. In particular, according to a recent study, small amounts of copper ions increase the growth rate of the tumor, so it is important to detect a low concentration of copper ions. A conventional method for detecting ions is a use of inductively coupled plasma (ICP) that is expensive and has a hassle things like preprocess of the samples and stabilization of the plasma which is necessary. However, the use of localized surface plasmon resonance (LSPR) is one of the techniques using the optics which can easily and quickly detect materials on the substrate in real time. The substrate and chelators, antibodies, aptamers or ligands are conjugated, can bind the desired materials like ions, proteins, even enzymes and genes. In this study, we made nanoplasmonic substrates using gold nanorods and D-penicillamine (DPA). D-penicillamine (DPA) is easy to conjugate with gold due to thiol group. But it is a chelator of the copper, so the DPA conjugated to the substrate is separated from the substrate and bonded to the copper II ions. Copper ion could be detected up to 100 picomolar (pM) concentration by using the nanoplasmonic substrate. It shows a unique selectivity for copper II ions. And we were able to detect copper in human blood-like environment. Based on this study, not only copper II ions but also other ions can be detected by making the substrate that can be more quickly and easily monitored for ions.
Yu Yan has completed her bachelor at the 21 years from South Medicine University of China and now she is a master whose major is histology and anatomy and from Medicine school of Jinan University. She has published 3 papers in reputed journals in 2016.
Dexamethasone (Dex) is widely used to treat chronic inflammatory diseases in the clinic. Increasingly, there is more attention being paid to the side effect of Dex. In this study, we investigated the involvement and mechanism of Dex exposure in accelerating mineralization during long bone formation. We first determined that Dex exposure could accelerate long bone mineralization in vivo, but there was no apparent difference between control and Dex-treated in the phalanges model in vitro. Next, we established that Dex exposure promoted angiogenesis in the chick yolk sac membrane (YSM) model. In addition, it increased HUVEC cell proliferation and migration in culture. We found that Dex could enhance angiogenesis when phalanxes were cultured on chick chorioallantoic membrane (CAM) and correspondingly increased the expression of angiogenesis-related genes in the phalanxes. Furthermore, we also revealed that Dex exposure reduced the number of osteoblasts and simultaneously increased the number of osteocytes in ex vivo cultured phalanges. Runx-2 and Col10α1 expressions were up-regulated by Dex exposure, indicating that Dex exposure accelerated the terminal differentiation of osteoblasts. Lastly, we demonstrated that MC3T3-E1 cells cultured in the presence of Dex accelerated their mineralization. In summary, we have shown that the ability of Dex to initiate angiogenesis is the mechanism that allows it to accelerate mineralization during long bone formation.