Oluwatobi S Oluwafemi
University of Johannesburg, South Africa
Title: Synthesis, antibacterial, cytotoxicity and sensing properties of starch-capped silver nanoparticles
Biography
Biography: Oluwatobi S Oluwafemi
Abstract
We herein report the antibacterial, cytotoxicity and sensing properties of dextrosed reduced starch-capped silver nanoparticles (Ag-NPs). The synthesis involves the use of water, starch, and dextrose which are environmentally benign materials as the solvent, stabilizing, and reducing agent respectively while AgNO3 was used as the silver precursor. The reaction was carried out without the use of any accelerator and the antibacterial activities of the Ag-NPs synthesised at different reaction time were investigated. The as-synthesised Ag-NPs were characterized using UV-vis absorption spectroscopy, Fourier transform infra-red spectroscopy (FTIR), Raman spectroscopy, X-Ray diffraction analysis (XRD) and High resolution transmission electron Microscopy (HR-TEM). Antibacterial study show that, all the as-synthesised Ag-NPs show good antibacterial activities against E coli and two strains of P. aeruginosa, which are antibiotic sensitive and resistant bacteria. In addition, the Ag-NPs produced at longer reaction time (48 h) showed a better antibacterial efficacy than those synthesised at lower reaction time. The cytotoxicity evaluation on Human THP-1 monocyte cell line indicated that the as-synthesised Ag-NPs are less toxic than AgNO3 at lower concentrations. The as-synthesised Ag-NPs are very sensitive towards hydrogen peroxide (H2O2) and show a linear response over a wide concentration range upto 10−10 M H2O2