Scientific Program

Conference Series Ltd invites all the participants across the globe to attend 2nd International Conference and Exhibition on Nanomedicine and Drug Delivery Tokyo, Japan.

Day 1 :

Keynote Forum

Hiroshi Maeda

Osaka University Medical School ,Japan

Keynote: Drug Delivery to Cancer based on Nano Medicine utilizing EPR effect

Time : TBA

NanoDelivery 2018 International Conference Keynote Speaker Hiroshi Maeda photo
Biography:

Hiroshi Maeda is a world renowned expert in macromolecular therapeutics. He created the world first polymeric-conjugate drug, SMANCS, approved for treatment of hepatoma in Japan. Consequently, he discovered the concept of EPR effect of macromolecular-drugs, ubiquitous mechanism for solid tumor selective targeting of polymeric drugs. He received MS, University of California, Devis, Fulbright student, Ph.D., M.D., Tohoku University. He published more than 450 papers in reputed journals. He was awarded Lifetime-Achievement-Award at Royal-Pharmaceutical-Society, Princess-Takamatsu-Award in Cancer Research, Tomizo-Yoshida-Award, highest award of Japan-Cancer-Assoc., and also selected as most cited influential scientist in pharmacology by Thomson Reuters 2014, and H-index of 89.

Abstract:

History of anticancer agents can be traced back 70 years, and that of photodynamic therapy (PDT), or boron / thermal neutron capture therapy (BNCT) to more than 100 years, or 50 years, respectively.  However, WHO or NCI of USA admit that most of cancer drugs developed were failure of >90%.  A major reason for these failures, is attributed to that there is no general principle to deliver the drugs to cancer tissue until the EPR (enhanced permeability and retention) effect was discovered in 1986 by us.  I will present in this meeting advantages of macromolecular drug or nanomedicine for cancer drug-targeting to tumor based on the EPR effect in solid tumor.  Also, I will talk about the history,  and controversial issues of the EPR effect, including various factors involved, such as heterogeneity, genetic mutational diversity, obstacles to the tumor blood flow or thrombi formation, and counter measures to overcome these problems in the EPR based drug delivery.  Also gaps between experimental models of mice, in contrast to practical human clinical setting, will be discussed.  Issues of cell internalization, which is greatly affected by the nature of active pharmaceutical ingredient (API) was demonstrated using HPMA-polymer(P)-conjugated-pirarubicin (THP) and P-doxorubicin (P-DOX), where P-THP was more than 30 fold better than P-DOX.  Critical importance of the enhancers of the EPR effect such as nitroglycerin are also discussed, and brief results of clinical pilot study of P-THP will be presented.

Keynote Forum

Veronique Preat

University of Louvain, Louvain drug research Institute

Keynote: Local and targeted delivery of nanomedicines for the treatment of glioblastoma

Time : TBA

NanoDelivery 2018 International Conference Keynote Speaker Veronique Preat photo
Biography:

Véronique Préat is full professor of pharmaceutics at the Faculty of pharmacy and head of the laboratory of Advanced drug delivery and biomaterials at the Louvain Drug research institute of the University of Louvain. She received a master and PhD in pharmaceutical sciences from this university. Her research area is focused on advanced delivery systems for unmet medical and pharmaceutical needs. In particular, her research on nanomedicines mainly focuses on the oral delivery of lipidic and polymeric nanoparticles loaded with drugs and the intravenous and local delivery of nanomedicines targeting the tumoral endothelium and cancer cells. She also focuses on the delivery of DNA and RNA with a particular interest in vaccination and cancer treatments. She supervised 38 PhD theses. She is author of more than 250 publications and book chapters with a h index 54 and more than 11500 citations. She is a highly cited researcher since 2015.

Abstract:

Glioblastoma (GBM) treatment includes, when possible, surgical resection of the tumor followed by chemoradiotherapy but the survival remains low mainly due to local recurrences. The local and targeted systemic delivery of anticancer drug-loaded nanomedicines to treat GBM after surgical resection of the tumor is a promising strategy. Among the strategies that have been adopted in the last two decades to find new and efficacious therapies for the treatment of GBM, the local delivery of chemotherapeutic drugs in the tumor resection cavity emerged. We developed two formulations of anticancer nanomedicines that can be injected perisurgically in the resection cavity of orthotopic GBM. Both PEG-DMA photopolymerisable hydrogel containing paclitaxel loaded nanoparticles and lauroyl-Gemcitabine lipid nanocapsules that spontaneously form a gel significantly improved the survival of the GBM-bearing mice. Another nanomedicine-based strategy could also improve GBM outcome. Targeted nanotheranostics are promising multifunctional system characterized by nano-size, possibility of surface functionalization, diagnostic and therapeutic capabilities. Due to the loss of BBB integrity in the GBM area, we showed that active targeting or magnetic targeting of SPIO/paclitaxel loaded nanoparticles enhanced the biodistribution of the nanoparticles in the brain and enhanced the survival time of GBM bearing mice after IV administration. The potential of other nanomedicine-based treatments of GBM will be discussed.

 

NanoDelivery 2018 International Conference Keynote Speaker Madhavan Nair photo
Biography:

Madhavan Nair is the Founding Chair and Distinguished Professor of the Department of Immunology at HWCOM, Florida International University. Dr. Nair’s main contribution to science include: a) first to report of reduced NK cell activity in intravenous drug users, b) first to report of immunoregulatory effects of HIV-1 recombinant peptides, c) first to report of differential effects of HIV-1B and C tat protein on secretion of neuropathogenic (IDO) and inflammatory molecules by primary monocytes and astrocytes, d) first report of morphine-induced apoptosis, e) synergistic effects of drug abuse and HIV-1 proteins on various immune responses and f) proteomic profiling of normal human astrocytes treated with cocaine, g) heroin induced differential protein expression by astrocytes h) transmigration of drugs bound nanocarrier across blood brain barrier model and i) the first report of transport and controlled release of HIV drugs bound to novel magneto- electric nanoparticles across BBB.

Abstract:

2014 report suggests that more than 36.9 million people are living with HIV/AIDS in the world today that includes more than 1.2 million people from US. Current studies also show that more than 247 million people are affected with substance abuse in the world that includes more than 24 million Americans. Reports also show that more than 3-4 million people are co-affected with HIV and illicit drug use. Although highly active anti-retroviral therapy (HAART) has resulted in remarkable decline in the morbidity and mortality in AIDS patients, inadequate delivery of HIV drugs across the blood-brain barrier (BBB) to the brain results in HIV persistence. Drugs of abuse such as opiates act synergistically with HIV-1 to potentiate the HIV-related neurotoxicity that leads to development of Neuro-AIDS. In recent years, use of nanotechnology has shown exciting prospect for development of novel drug delivery systems. We herein report the development of a Magneto-Electric Nanocarrier (MEN) to deliver and release on demand of HIV drugs and opiate antagonist, which are otherwise impenetrable to brain and inhibit HIV and reverse opiate mediated adverse neurological effects. The proposed nanocarrier is anticipated to simultaneously reduce Neuro-AIDS and opiate addiction in HIV-1 infected opiate addicts. Further, this invented/patented new technology will have universal applicability for targeting and controlled release of drugs against a variety of other CNS diseases such as Parkinson’s, Alzheimer’s, brain tumors etc

Keynote Forum

Arvydas Tamulis

Institute of Theoretical Physics and Astronomy of Vilnius University,Lithuania

Keynote: Possible to Excite Transmission of Nerve Signals in Brain for Cancer Therapy

Time : TBA

NanoDelivery 2018 International Conference Keynote Speaker Arvydas Tamulis photo
Biography:

Arvydas tamulis has completed his Ph.D. degree of the Theoretical and Mathematical Physics in Vilnius University in 1985.He worked has a Research Fellow at the Institute of Physics of the Lithuanian Academy of Sciences from 1985-1996.At Present he is working as a Senior researcher at the Institute of Theoretical Physics and Astronomy, Vilnius University. Total number of scientific publications 228: 5 chapters of books, 117 scientific articles in the refered issues (67 of them in the ISI Web of Science list with the impact factor). Science presentations in more than 100 conferences and symposia in Lithuania, Russia, Poland, England, Wales, Scotland, Germany, Japan, Denmark, Norway, Sweden, Italy, Spain, France, Netherlands and USA. During 22 years of restored Lithuanian independancy have sucessfuly participated in 27 scientific projects.

Abstract:

Neutral radical acetylcholine molecule (ACh) play important role in the transmission of peripheral nerve signals and in the processes of the central nervous system which are related with consciousness but also might be employed for the therapy of brain and other nervous system tumors.
Molecular complex containing two ACh molecules and photoactive hypericin molecule (see in the center of Figure) in acetonitrile or in water molecules environments were investigated using quantum mechanical various density functional methods.The neutral radical ACh molecule is not regular, because its nitrogen atom possesses four chemical bonds with carbon atoms (see in the right of Figure), even though it typically forms only 3 single bonds therefore ACh molecule possesses one single unpaired electron spin.
During 2002-2004 years research efforts at Los Alamos National Laboratory (LANL) focused on constructing a quantum computer based on regular arrays build from neutral radical molecules possessing one single unpaired electron spin. The idea was built on the ability to manipulate individual electron spins in some kind of a solid matrix or lattice1-3. It was suggested that self-assembled monolayer systems could be used to create a macroscopic ensemble of quantum entangled 3-spin groups as a first step in quantum information processing4,5. The spins of such a group could be connected by dipole–dipole quantum couplings. Application of a non-uniform external magnetic field could allow selective excitation of every spin inside the group. The proper sequence of resonant electromagnetic pulses would then drive all spin groups into a 3-spin entangled state. In the approach suggested in Ref. [1], the spins were to be associated with a single unpaired electron spin of a neutral radical molecule in the self-assembled monolayer.group could be connected by dipole–dipole quantum couplings. Application of a non-uniform external magnetic field could allow selective excitation of every spin inside the group. The proper sequence of resonant electromagnetic pulses would then drive all spin groups into a 3-spin entangled state. In the approach suggested in Ref. [1], the spins were to be associated with a single unpaired electron spin of a neutral radical molecule in the self-assembled monolayer.
We have found self-assembly of four neurotransmitter ACh molecular complexes in a water molecules environment by using geometry optimization with DFT B97d method. These complexes organizes to regular arrays of ACh molecules possessing electronic spins, i.e. quantum information bits6,7.
These spin arrays could potentially be controlled by the application of a non-uniform external magnetic field and by attaching the molecules to the ACh arrays with proper choosing parameters of g-tensor8,9. The proper sequence of resonant electromagnetic pulses would then drive all the spin groups into the 3-spin entangled state and proceed large scale quantum information bits.
Calculations by using time dependent density functional methods PBE0 and PBEh1PBE with basis set TZVP shows that maximum of excitation by light should be in the region 660-650 nm depending on various molecules environments. That allow to excite transmission of nerve signals in brain or other nervous systems for cancer therapy.

Keynote Forum

Ling Peng

French National Scientific Research Center (CNRS),France

Keynote: Charm of dendrimer nanotechnology for biomedical applications

Time : TBA

NanoDelivery 2018 International Conference Keynote Speaker Ling Peng  photo
Biography:

Ling Peng is a leading expert in developing functional dendrimer nanosystems for drug delivery in biomedical applications. She has successfully established bio-inspired structurally flexible and self-assembling dendrimer nanosystems for drug and nucleic acid delivery. One of the dendrimers developed by her team has been scheduled in clinical trials. Dr Peng has coordinated and participated in different European projects and actively involved in several European CAOST Actions. She is currently a research director at the French National Scientific Research Center (CNRS), and a principle investigator at the Centre Interdisciplinaire de Nanoscience de Marseille (CINaM). Her research team has been labelled by La Ligue contre Le Cancer in France for the period of 2016-2020, and she was awarded for the Prize of Dr and Mrs Henri Labbé by the French Academy of Sciences in 2017.

Abstract:

Nanotechnology is widely expected to bring breakthroughs in specific delivery of the right therapeutic agent to the right patient at the right disease lesion. Dendrimers are ideal nanocarriers for drug delivery by virtue of their uniquely well-defined structures and multivalent cooperativity confined within a nanosized volume per se. We have established bioinspired structurally flexible and self-assembling supramolecular dendrimers for drug delivery.1-6 These dendrimers are excellent nanocarriers for personalized medicine: they are able to form modular, responsive and adaptive nanosystems, and effectively deliver various chemo- and bio-therapeutics as well as imaging agents for precise diagnosis and personalized treatment in various disease’s models. These studies have offered new perspectives in dendrimer nanotechnology based biomedical applications.

Keynote Forum

Michal M. Godlewski,

Warsaw University of Life Sciences WULS-SGGW,Poland

Keynote: New biocompatible oxide nanoparticles as carriers of bioactive compounds through the blood-brain barrier

Time : TBA

NanoDelivery 2018 International Conference Keynote Speaker Michal M. Godlewski, photo
Biography:

Dr Michal M. Godlewski is currently the Vice-Dean for International Studies at the Faculty of Veterinary Medicine, WULS-SGGW. He defended the PhD in 2003 and received habilitation in 2015. Currently he manages Cytometric Laboratory of the Department of Physiological Sciences and Laboratory of Nanotechnology and Nanoengineering in the Veterinary Research Centre/Centre for Biomedical Research of the Department of Large Animal Diseases with Clinic. Dr Godlewski is recognized expert in the field of nanoparticle applications for medicine. In his research he collaborates with 20 scientific institutions and 6 business partners. His recent scientific interest relate to the interactions of nanomaterials with living organism and the development of nanoparticles for bio-medical applications. He is author/co-author of 60 papers in the WoS database and over 10 chapters in academic monographies, cited over 500 times. Forty invited talks and 30 national and international prizes and medals for innovation, reflect the relevance of his research.

Abstract:

Blood-brain barrier is major obstacle for drug delivery to the brain. In this study we focused on oxide nanoparticles (NPs) as potential drug carriers. Mice received suspension of Y2O3:Tb:Lectin NPs (10mg/ml; 0.3ml/mouse) via gastric gavage (IG) and were sacrificed after 24h, 48h and 1 week. Control group received equivalent suspension of pure lectin. All protocols were conducted according to EU guidelines and approved by LEC agreement No 44/2012. Following the sacrifice, brain tissue was collected for the analyses under confocal microscope and scanning cytometry. Lectins were chosen as a perfect model substance for the use of NPs as carriers, due to the fact that physiologically they are not absorbed from the gastrointestinal tract.

Control group exhibited extremely low signal for lectin not exceeding background level. In the group which received Y2O3:Tb:Lectin, signal for lectin coincided with NPs red fluorescence in the brain as soon as 24h after IG. Following 48h, the convergence lowered and after 1 week only free lectin were observed in the brain tissue.

In conclusion, oxide NPs proved able to transport bioactive compounds through the blood-brain barrier. After entering brain tissue complexes of nanoparticles and lectin dissolved and free lectin was deposited in the tissue.

  • Advanced Nanomedicine
Location: TBA

Session Introduction

Kesavan Karthikeyan

Guru Ghasidas Central University,India

Title: Chitosan Based Positively Charged Mucoadhesive Nanocarriers for Ocular Drug Delivery
Speaker
Biography:

Dr. K. Kesavan is a well-versed nanotechnologist, with expertise in developing nanoparticulate systems for delivering lipophilic drugs to eye for the treatment of ocular diseases. His group developed HP-β-CD-based mucoadhesive pH induced hydrogel system and mucoadhesive microemulsion of dexamethasone for ocular delivery to treat uveitis in rabbit eye model. His research group recently studied the ion activated hydrogel system and phase transition microemulsion for ocular delivery to treat keratitis in rabbit eye model. Recently his group published a paper in ocular selfmicroemulsifying drug delivery system of prednisolone for effective treatment of uveitis in rabbit eye model. Currently, his group working on the development of the nanoparticulate drug delivery systems for ocular drug delivery, which would offer the advantages of decreased dose and dosage regimen, sustained release, improved patient compliance, and enhanced safety profile.

Abstract:

Cornea and conjunctiva carry a negative charge upon their surface, due to the presence of negatively charged mucus residues on the outer side of their membranes, and to selective active ion pumps. Ocular surface should thus be selective to positively charged delivery systems that interact with cells, leading to increased drug permeability and prolonging the pharmacological effect. Chitosan (CS), as a unique positively charged polysaccharide, has been one of the most popular biopolymers for development of drug delivery systems for various applications, due to its promising properties, including high biocompatibility, excellent biodegradability, low toxicity, as well as abundant availability and low production cost. In fact, an ionic interaction between the positively charged amino groups of CS and negatively charged sialic acid residues in mucus has been proposed as the mucoadhesion mechanism. Since last decade, increasing attention has been attracted by delivery systems fabricated from natural biopolymer-based polyelectrolyte complexes (PEC), formed by electrostatic interactions between two oppositely charged biopolymers. The preparation of nanocapsule via complex coacervation method is based on the PEC through the electrostatic interactions between cationic and anionic polymers, resulting in the formulation of insoluble spherical beads or capsules. Due to the protonation of amino groups on the backbone, CS becomes a cationic polyelectrolyte in acidic medium, which could form PEC with negatively charged polyelectrolytes, resulting in various applications. In recent years, positively charged liposomes and nanoemulsions have been used as drug carriers. CS is suitable for formulation of mucoadhesive cationic nanoemulsion because it is positively charged, making it able to adhere to the negatively charged oil globules in nanoemulsion and is soluble in diverse acids and able to interact with polyanions to form complex and nanogel. This is an effort to summarize the recent developments in the area of CS based cationic mucoadhesive nanocarriers for ocular drug delivery.

Speaker
Biography:

Virendra Gajbhiye completed his M. Pharm and Ph.D. from Dr. H. S. Gour University, Sagar, India. He was a postdoctoral research fellow at Wisconsin Institute for Discovery, University of Wisconsin, Madison, WI, USA and Department of Biomedical Engineering, Oregon Health and Science University, Portland, OR, USA. Currently, he is working as a Scientist at Nanobioscience Group, Agharkar Research Institute, Pune, India. Virendra Gajbhiye has his expertise in developing multifunctional nanocarriers and their use in nanomedicine and theranostics, targeted delivery of drug/siRNA/miRNA for cancer therapy, and tissue engineering. He has extensively worked on drug targeting to brain. Currently, he is extensively working on delivery of siRNA/miRNA for treatment of breast cancer.

 

Abstract:

Cancer is one of the major health problems worldwide and the number of cancer cases is projected to increase by 50% by 2030. Current therapies include surgery, chemotherapy, radiation and hormonal therapy which are also used sometimes in combination to lower the risk of reoccurrence. These therapies used till date are followed by various side effects and disadvantages. Hence, newer and targeted therapies are required. One of the ways is to explore siRNA therapy that works on RNA interference mechanism. siRNA of 21-25 nucleotide long help in turning off expression of cancerous gene by directing degradation of selective mRNA. The major bottleneck in siRNA therapies is their delivery to desired cell type. siRNA does not readily cross the cellular membrane because of their negative charge and size. Moreover they are very sensitive to nuclease degradation, thus posing serious challenges in efficient delivery. Nanotechnology has been drawing a huge attention for successful and targeted delivery of siRNA. Hence we thought of using nanotechnology based vector which can be used to deliver siRNA efficiently. Multifunctional cationic dendrimers, mesoporous silica nanoparticles (MSNPs) and other NPs are being used by use to deliver siRNA specifically to breast as well as prostate cancer cells. Our study shows that a unimolecular dendritic system (Figure 1) conjugated with luteinizing hormone releasing hormone (LHRH) analogue was able to deliver siRNA specifically to LHRH receptor overexpressing breast as well as prostate cancer cells. In one of our recent study, we observe that siRNA delivery can be achieved via unimolecular dendrimers conjugated with peptide by targeting integrin receptors of breast cancer cells. Also, cationic cobalt ferrite nanoparticles synthesized in our lab was able to successfully deliver siRNA in breast cancer cells. Similarly, our latest results show that multifunctional MSNPs (Figure 2) can be effectively use for the treatment of drug resistant breast cancer cells.  

  • Nanomaterials for drug delivery
Location: Hilton Tokyo Narita

Session Introduction

Nana Zhao

Beijing University of Chemical Technology, China

Title: Versatile organic/inorganic nanohybrids for delivery systems
Biography:

Prof. Nana Zhao has her expertise in strategic design, controlled synthesis, and biomedical applications of organic/inorganic nanohybrids, including diagnosis of diseases, gene delivery, controlled drug-release, and imaging. Integrating the control over morphology, surface functionalization, and self-assembly strategies, the performance of nanohybrids could be improved further.

Abstract:

Organic/inorganic nanohybrids with favorable physical and chemical properties constructed form suitable surface functionalization of inorganic nanoparticles (NPs) with superior polycations are promising candidates as carriers with multi-functions. The morphology (size and shape) of NPs are considered to have an intense influence on their interaction with cells and biological systems, while the effect of morphology on gene carriers are poorly understood. We developed several facile strategies to construct organic/inorganic nanohybrids of polycations and inorganic nanoparticles. Grafting-from, grafting onto and host-guest interactions were all utilized for the fabrication of nanohybrids. Furthermore, we employed SiO2 and Au NPs as model systems to investigate the morphology effect. A series of novel gene carriers based on polycation-functionalized SiO2 and Au NPs with different morphologies were designed and synthesized, including nanospheres, nano-octahedras, nanorods, arrow-headed nanorods and chiral nanorods, et al. The morphology of both SiO2 and Au NPs is demonstrated to play an important role in gene transfection. Based on the results, star-shaped hollow silica carriers with photothermal gold caps were synthesized for the co-delivery of drugs and genes. One dimensional nanohybrids of polycations and iron oxide or quantum dots were also designed and satisfying therapeutic effects were achieved. In addition, magnetic resonance (MR) or fluorescence imaging could be realized in the same nanostructure. Therefore, combing the intriguing properties of inorganic parts, the carriers could integrate the functions of imaging and be employed for theranostic platforms. These results may provide new avenues to develop promising carriers and useful information for the application of NPs in biomedical areas.

Arvydas Tamulis

Vilnius University Institute of Theoretical Physics and Astronomy,Lithuania.

Title: Quantum Entanglement Communications in Photoactive Synthetic Bio-Systems and in Neural Networks
Speaker
Biography:

Abstract:

Together with my collaborators I have been investigating the self-assembly of molecules that result in supramolecular bioorganic and minimal cellular systems, as well as the biochemistry of these assemblies. The self-assembly and biochemistry depend on quantum mechanics laws which induce hydrogen and Van der Waals bondings [1–10]. Therefore our work has been done through modelling based on quantum mechanical time dependent density functional theory, which also makes it possible to study quantum entanglement in such systems (TD-DFT).
In the work presented here, quantum entanglement takes the form of a quantum superposition of the active components in synthesized self-assembled and self-replicating living systems. When a quantum calculation of an entangled biosystem is made that causes one protocell photoactive biomolecule of such an entangled pair to take on a definite value (e.g., electron density redistribution tunnelling or electron spin density redistribution tunnelling), the other protocell photoactive biomolecule of this pair will be found to have taken the appropriately correlated value (e.g., electron density redistribution tunnelling or electron spin density redistribution tunnelling) in two quantum entangled excited states of this bicellular system (see Figure 1). In our simulations, the starting separation distance of the supramolecular bio systems changed during geometry optimization procedures, taking on final values that mimic those associated with real-world intermolecular interaction processes. Furthermore, the modelling indicates that quantum entanglement occurs between the prebiotic subsystems which enhances the photosynthesis of the combined systems. The enhancement occurs because two additional quantum entangled excited states are created through the simultaneous excitation of the combined system’s two prebiotic kernels or. two protocells. The additional photosynthesis made possible by the quantum entanglement potentially provides a selective advantage through an enhancement of usable energy leading to faster growth and self-replication of minimal living cells [3-7], which in turn can lead to accelerated evolution.
Living systems that are self-assembled and self-replicating exist in wet and warm environments where stochastically moving supramolecular subsystems continuously generate and destroy quantum entangled states by non-equilibrium effects. While no static entanglement exists, quantum entanglement nonetheless temporarily occurs amongst the biomolecules inside the combined system or between the living subsystems, i.e. between two protocells or two prebiotic kernels [3, 4].
This warm quantum coherence is proposed by others as a basis for DNA stability and for the understanding of brain magnetic orientation during migration in more than 50 species of birds, fishes and insects [4]. Experimental evidence also exists for quantum-coherence as a basis for more efficient light-harvesting in plant photosynthesis [4]. Furthermore, quantum entanglement exists between supramolecules used in the sense of smell and in the microtubules of brain neurons where it gives rise to critical quantum vibrations [6].
Using quantum mechanical investigations, we have now started to design quantum entanglement communication molecular logical devices which hold promise for use in nano-medicine biorobots to fight molecular diseases such a cancer tumors, and against the new kinds of synthesized microorganisms and nano guns [4, 5, 9, 10].
Our current research concerns implementation of liquid state quantum information processing based on spatially localized electronic spin in the neurotransmitter stable acetylcholine (ACh) neutral molecular radical. Using DFT quantum calculations we proved that this molecule possesses stable localized electron spin, which may represent a qubit in quantum information processing. The necessary operating conditions for ACh molecule are formulated in self-assembled dimer and more complex systems. The main quantum mechanical research result is that the neurotransmitter ACh systems, which were proposed, include the use of quantum molecular spintronics arrays to control the neurotransmission in neural networks [11-13].

Julien Bras

University Grenoble Alpes,France

Title: Nanocellulose alginate composite for 3D cell growth
Speaker
Biography:

Dr Julien Bras (H index: 32; 122 scientific papers, 11 patents) is Associate professor at Grenoble Institute of Technology (Grenoble INP Pagora). He is member of Institut Universitaire de France (IUF, 2016-2021). He is deputy director of LGP2 (Laboratory of Pulp & Paper Science) and head of the “Multiscale Biobased Material” group (ab. 40 pers.). He has directly supervised 23 PhD and 14 Post-doc focusing on research on Biomaterials, Nanocellulose & Specialty papers. After engineer diploma in Chemistry and a PhD on Renewable Materials in 2004, he worked in industry few years as Innovation Manager within Ahlstrom Specialty paper and then left industry to become associate professor since 2006.

Through his different experiences, he develops several competences. His expertise deals particularly with Nanocellulose, biobased and smart materials. More precisely, he proposed new way of production, characterization and functionalization of nanocellulose for several applications since more than 10 years. He has already coordinated or supervised several industrial and European projects in FP6, FP7 and Marie-Curie calls. He is involved in a Labex & Carnot institute organization as WP leader and is associate editor for an Elsevier Journal (Industrial Crops and products).

Abstract:

Dr Julien Bras (H index: 32; 122 scientific papers, 11 patents) is Associate professor at Grenoble Institute of Technology (Grenoble INP Pagora). He is member of Institut Universitaire de France (IUF, 2016-2021). He is deputy director of LGP2 (Laboratory of Pulp & Paper Science) and head of the “Multiscale Biobased Material” group (ab. 40 pers.). He has directly supervised 23 PhD and 14 Post-doc focusing on research on Biomaterials, Nanocellulose & Specialty papers. After engineer diploma in Chemistry and a PhD on Renewable Materials in 2004, he worked in industry few years as Innovation Manager within Ahlstrom Specialty paper and then left industry to become associate professor since 2006.

Through his different experiences, he develops several competences. His expertise deals particularly with Nanocellulose, biobased and smart materials. More precisely, he proposed new way of production, characterization and functionalization of nanocellulose for several applications since more than 10 years. He has already coordinated or supervised several industrial and European projects in FP6, FP7 and Marie-Curie calls. He is involved in a Labex & Carnot institute organization as WP leader and is associate editor for an Elsevier Journal (Industrial Crops and products).

Speaker
Biography:

Dr Zi (Sophia) Gu is a Lecturer and National Health and Medical Research Council (NHMRC) Early Career Fellow in the School of Chemical Engineering at University of New South Wales (UNSW), Sydney. She obtained PhD from Australian Institute for Bioengineering and Nanotechnology at The University of Queensland (UQ), Australia, in 2011, and she was awarded a NHMRC EC Fellowship in 2013 when she started her independent research at UQ prior to joining UNSW in 2016. Sophia has been working in multidisciplinary research areas that combine the knowledge and skills across nanotechnology and biomedicine, and has developed a targeted anti-restenotic therapy and built inorganic nanoparticle platforms for diagnosing and treating diseases. She published in high-impact journals including Advanced Materials, Biomaterials, Chemistry of Materials, Materials Horizons, and Journal of Controlled Release etc. Her recent awards include The Monash Engineering Women’s Leadership Award and Deputy Vice-Chancellor (Academic) Award. Her research group focuses on developing multifunctional nanomaterials for theranostic application (disease therapy and diagnosis).

Abstract:

Recent progress in material chemistry has enabled scientists to combine therapeutic and diagnostic agents on a single nanoplatform, which is designated as theranostics [1]. However, it remains a challenge to achieve theranostics with safety and high performance. We utilised a clay nanosheet LDH as the carrier of Mn(II) and anti-cancer drug 5-FU, and synthesised a new nanocomposite with outstanding pH-ultrasensitive T1-MRI performance and enhanced drug delivery efficiency [2,3] (Figure 1). Mn(II) element was doped in LDH nanostructure by isomorphic substitution to synthesis Mn-LDH nanosheets. The generated Mn-LDH (~50nm) showed an ultrahigh T1-weighted relaxivity of 9.48 mM-1s-1 at pH 5, possibly caused by the unique microstructure of Mn ions in Mn-LDHs. The relaxivity of Mn-LDH was highly sensitive to pH, which increased sixfold when pH was reduced from 7.4 to 7.0. The Mn-LDH was effectively internalised in HT29 cells, and the anti-cancer efficiency of 5-FU was enhanced by LDH delivery (IC50=1.54 µg/ml). The in vivo MRI evaluation showed an accumulation of Mn-LDH in tumour compared with liver and kidney, and the MRI signal maintained in tumour for 72 h. The second part of this talk will present a [email protected] nanocomposite as a pH-sensitive T1/T2 dual modal MRI contrast agent, hyperthermia agent and efficient drug carrier. The nanocomposite showed T1-weighted relaxivity increased from 1.88 to 6.23 mM-1s-1 and T2-weighted relaxivity from 226.18 to 367.25 mM-1s-1. This pH-sensitive and high relaxivity is not found in SPION as T2-MRI contrast agent. The [email protected] nanocomposite also has mild hyperthermia and efficient drug delivery properties.

Speaker
Biography:

Mahdieh Heydarigoojani has completed her bachelor at the age of 23 years from Science and Research islamic azad university in Iran and she is master student of biomedical engineering at TU University of Vienna. This reaserch has been done by collaboration with Tarbiat modares University of Iran .

Abstract:

In Atherosclerosis, vessels wall getting narrow and stiff. It is refers to buildup LDL(low density lipoprotein) and as a result, blood and nutrition can not pass through artery. Also Endothelial cells are injured and smooth muscle cells immigrate into the Intima. Therefore anticancer drugs would be appropriate choice for treatment. Curcumin and Docetaxel showed anti inflammatory and anti proliferative effects respectively. But their clinical application has been limited due to the fact that hydrophobicity and poor bioavailability. For this reason a potent mPEGPCL micelle system was synthesized and release profile of Curcumin and Docetaxel from drug- loaded micells was characterized.

Speaker
Biography:

Dr. Tomitaka received Ph.D. in Electrical and Computer Engineering from Yokohama National University, Yokohama, Japan in 2011. She was a Japan Society for the Promotion of Science (JSPS) postdoctoral fellow at Kyoto University, Japan from 2011 to 2012, and a JSPS Postdoctoral Fellow for Research Abroad at University of Washington, USA from 2012 to 2014. She is currently a Postdoctoral Associate in the Department of Immunology, Florida International University (FIU). Her expertise is magnetic engineering and nanotechnology for biomedical applications

Abstract:

 

Drug delivery systems have shown promising results for various diseases owing to the development in nanotechnology. However, the therapeutic effect is still limited due to the lack of monitoring system after drug administration. Image-guided drug delivery is an emerging strategy which has a great potential to improve the therapeutic efficacy of drug delivery systems by adding an image guidance. The addition of imaging enables monitoring of drug distribution after administration and quantification of the drugs at target site. Among various nanoparticles developed for biomedical applications, magnetic nanoparticles have shown promise for brain targeting and magnetic resonance imaging (MRI) due to their unique magnetic properties. Gold nanoparticles possess excellent properties for various imaging modalities including X-ray computed tomography (CT) and photoacoustic imaging. Combining magnetic and plasmonic features in a single nano-structure gives multi-functionality which is suitable for image-guided drug delivery.

In this study, we synthesized magneto-plasmonic nanocarriers by a two-step process. First, magnetic nanoparticles (MNPs) were synthesized by co-precipitation, followed by gold coating of MNPs by citrate reduction. The spherical magneto-plasmonic nanoparticles with the surface plasmon resonance (SPR) peak in the visible range were synthesized in this step. In the second step, the morphology of the nanoparticles was modified to star shape to adjust SPR within the near infrared (NIR) range. This NIR responsiveness is suitable for optical imaging systems due to the long penetration of NIR light inside body. A transmission electron microscopy (TEM) study showed successful synthesis of star shaped nanoparticles. We conducted magnetic and optical characterization of the magneto-plasmonic nanocarriers, and confirmed superparamagnetic property and SPR peak within NIR region. These results indicate the capacity of magneto-plasmonic nanocarriers for drug delivery systems with highly precise image guidance using multiple imaging modalities.

Speaker
Biography:

Hippolyte Durand is doing its PhD in Univ. Grenoble Alps at LGP2 (Laboratory of Pulp and Paper Science and Graphic Arts) located in Grenoble, France. He is has a strong background in cellulose and nanocellulose science. He is part of the CELLICAL project which aims at developing new medical devices for wound healing. The use of cellulose nanofibrils is the corner stone of the research done by the consortium of CELLICAL project.

Abstract:

For several decades now, Cellulose Nanofibrils (CNF) from wood are of high interest in many research fields. They turned out to have high specific area, low density, excellent mechanical properties and chemical reactivity, besides their character of renewable resource. Highly entangled networks of such nanofibrils (5-20nm in width and 1-5µm long) are formed easily, resulting in films with high mechanical and barrier properties that make them interesting candidates for a number of applications, as seen recently in the medical field. The surface functionalization of these nanofibrils allows researchers to bring new properties to this wonderful material in order to incorporate it in new innovative bio-based products. The purpose of this work is to use CNF as a drug carrier in order to develop bioactive medical devices. CNF surface were first functionalized with drugs either by covalent or non-covalent binding and then incorporated in a matrix of collagen based material or used as films. Both types of samples, composites and films, are produced by solvent casting in controlled conditions. The drug release study of these medical device prototypes was conducted in-vitro in different model release medium such as PBS and enzyme containing solutions and gels. The use of CNF as drug carrier permitted to extend the duration of the drug release, resulting in an innovative and controlled drug delivery. This project intends to bring new bio-active products to the market of medical devices in order to ease post-surgery treatments and improve wound healing

  • Synthesis of Nanoparticles for Drug Delivery
Location: Hilton Tokyo Narita

Session Introduction

Shubiao Zhang

Dalian Minzu University ,Chima

Title: Cationic lipids for gene delivery in vitro and in vivo
Speaker
Biography:

Dr. Shubiao Zhang now is a distinguished professor at Dalian Minzu University and a doctor supervisor at Dalian Institute of Chemical Physics and Dalian University of Technology. He is involved in the study about non-viral vectors for gene delivery and bio-active materials. He got many awards at the university including Young Backbone Teacher of Liaoning Province, New Century Excellent Talents of Ministry of Education, Academic Paper Award of Liaoning Province and Scientific and Technological Paper Award of Dalian City. He leads many research programmes supported by Natural Science Foundation of China, Ministry of Education, Hoffmann La-Roche Ltd. and some domestic companies. He published over 80 peer-reviewed articles, among them over 40 were indexed by SCI, 15 by EI with a total cites of over 1200 times. He is a reviewer for many high quality peer-reviewed journals such as Journal of Controlled Release, Bioconjucate Chemistry, Molecular Pharmaceutics and Interational Journal of Pharmaceutics.

Abstract:

Several novel tri-peptide cationic lipids were designed and synthesized for delivering DNA and siRNA. They have tri-lysine and tri-ornithine as headgroups, carbamate group as linker and 12 and 14 carbon atom alkyl groups as tails. These tri-peptide cationic lipids were prepared into cationic liposomes for the study of the physicochemical properties and gene delivery. Their particle size, Zeta potential and DNA-binding were characterized to show that they were suitable for gene transfection. The further results indicate that these lipids can transfer DNA and siRNA very efficiently into NCI-H460 and Hep-2 tumor cells. The selected lipid, CDO14, was able to deliver combined siRNAs against c-Myc and VEGF for silencing distinct oncogenic pathways in lung tumors of mice, with little in vitro and in vivo toxicity

Speaker
Biography:

Janet Tan Sui Ling has completed her Master of Pharmacy (Mpharm) in 2015 from University of Bath, United Kingdom with first class honours. She was also awarded Royal Pharmaceutical Society Prize for Best Overall Third Year Student (2014), Pfizer Prize for Best Medicine Design in Third Year (2014), Vectura Prize for Best Advanced Drug Delivery (2015) and AstraZeneca Prize for Best in Cancer (2015). She contiuned her studies in PhD in University of Nottingham Malaysia Campus in 2015 and currently, is in her second year of study. She has won the Best PGR presenter in UNMC PG Link ’17 Conference. 

Abstract:

Oral delivery of amphotericin B (AmpB) remains challenging due to physicochemical properties of AmpB such that it results in meagre bioavailability (0.3%). In an advanced formulation, 1) nanostructured lipid carriers (NLC) were formulated as they can accommodate higher levels of cargoes and restrict drug expulsion and 2) a mucoadhesion feature was incorporated to impart sluggish transit along gastrointestinal tract and hence, maximize uptake and improve bioavailability of AmpB. Chitosan-coated and naked AmpB-loaded NLC formulations were successfully formulated. Physical properties of the formulations; particle size, zeta potential, encapsulation efficiency and mucoadhesion as well as the effect of the variable pH on the integrity of the formulations were investigated. The particle size of freshly prepared AmpB-loaded NLC was 163.1 ± 0.7 nm, with a negative surface charge and remained essentially stable over 120 days. Adsorption of chitosan caused a significant increase in particle size to 348.0 ± 12 nm with the zeta potential reverts towards positivity. The incorporation of chitosan increased the encapsulation efficiency significantly by 5%, to 99.3 ± 0.15% and observed lower expulsion of AmpB after exposure to variable of pH conditions. The mucoadhesiveness of the ChiAmpB NLC formulation was observed in both acidic (pH 5.8) and near-neutral pH (pH 6.8) conditions as opposed to AmpB-loaded NLC formulation. Hence, the incorporation of chitosan into the NLC formulation did not only impart mucoadhesive property but also protected against the expulsion of AmpB which makes it well-primed as a potential oral delivery system for AmpB.

Speaker
Biography:

Sakthivel Lakshmana Prabu has his expertise in Herbal formulations, nanoparticulate drug delivery system and its therapeutical applications. He is Having 17 years of professional experience with good reputed pharmaceutical industrial expertise in quality assurance, validation and formulation development.  He filled 3 international patents, published 93 research articles in International and National journals. Contributed 12 book chapters and edited 2 books. Book chapter entitled “Extraction of Drug from the Biological Matrix: A Review” has been downloaded 22,000 times worldwide. As a Principal Investigator, he received a project grant from Department of Biotechnology, India for the worth of 67 Lakhs. He is serving as reviewers for eight reputed journals and an editorial board member for five journals

Abstract:

Almost 80% of the adolescent populations of the world are affected with Acne vulgaris specifically during their puberty stage of life. Bacteria develop antibiotic resistance on repeated treatment with particular antibiotics by forming a rapport between the bacteria and antibiotic. In olden days, plants were used as a medicine for several diseases. Development of formulation for the treatment of acne is a major focused research in pharmaceutical sciences especially, in cosmetic care industries. The objective of our research is to prepare silver nanoparticles from the leaf extract of Ocimum Gratissimum and develop topical herbal gel formulation for the effective treatment of acne. Based on the research literatures, the medicinal plant Ocimum gratissimum is selected due to enormous pharmacological activity in relevance to acne. Methanolic leaf extract was prepared and analyzed for its phytoconstituents. The results of the study revealed the presence of phytoconstituents like alkaloids, flavonoids, tannins and saponins in the extract. 1mM aqueous solution of silver nitrate was used with the methanolic extract of Ocimum gratissimum for the preparation of silver nanoparticles. Formation of silver nanoparticles was confirmed by UV spectroscopy. Synthesized silver nanoparticles were spherical in shape with average particle size of 207.6 nm and the polydispersity index was found to be 0.256. These synthesized silver nanoparticles were incorporated into the gel base using Carbopol 934 and HPMC (1%w/v) and evaluated for its physicochemical properties and antibacterial activity. The results demonstrated that the developed topical herbal gel had easy washability, good spreadability and pH was found to be 6.72 and 6.80. Antibacterial study of the developed formulation showed higher inhibitory activity against Propionibacterium acne, Staphylococcus aureus and Escherichia coli when compared to the extract. The results of our study concluded that silver nanoparticle of Ocimum gratissiumum in aqueous gel base may be used for the treatment of acne vulgaris.

Speaker
Biography:

Andra-Sorina Tatar has received her BSc in Biochemistry and in Physics and the MSc in Molecular Biotechnology from the Babes-Bolyai University, Cluj-Napoca, Romania. Having an interdisciplinary background, she started her PhD work focusing on the design of antibody-conjugated nanoparticle-based systems and their application for the spectroscopic detection of circulating tumor cells and treatment of hematological diseases

Abstract:

Acute Lymphoblastic Leukemia (ALL) is the first and second most common malignancy in children and adolescents. With a broad range of genetic causes, this disease may appear with various protein expression profiles, routing the researchers towards a more promising patient-specific treatment plan.

Through our work, we aim to develop a nano-tool which is targeted towards the ALL specific CD19 surface protein via the strong antibody-antigen interaction. Specifically, we developed a gold nanourchin based vehicle for antiCD19 using a surfactant free seed mediated synthesis protocol. By employing the Trypan Blue dye exclusion method, we show the highly significant cytotoxic effect of our targeted particles against CCRF-SB B-lymphoblastic leukemia cells as compared to the non-targeted ones, as well as to the free antibody molecule. Cell cycle analysis using Flow Cytometry, metabolic activity investigation by MTS assay, and morphological analysis by TEM contribute with valuable additional insights to the conclusion that the antiCD19 targeted nanourchins accomplish their therapeutic duty. Additionally, these anisotropic nanoparticles possess interesting optical properties such as plasmon resonances in the NIR domain and the efficient amplification of the nearby electromagnetic field, which provide them with valuable imaging features and the capability of being used as spectroscopic tags inside living cells.

 

Acknowledgement: This work was supported by the project PN-II-RU-TE-2014-4-2426.

  • Smart Drug Delivery Technology
Location: Hilton Tokyo Narita

Session Introduction

Ajeet Kaushik

Florida International University, USA

Title: Non-invasive delivery of magnetic drug-nano-carrier to the brain
Speaker
Biography:

Ajeet Kumar Kaushik, Ph.D. (http://akaushik3.wixsite.com/nanocare), is an Assistant professor at the Centre of Personalized Nanomedicine, Institute of NeuroImmune Pharmacology, Department of Immunology of Florida International University, USA. He is recipient of various reputed awards and exploring smart electrochemical sensing systems for rapid diagnostics and nanocarriers for on-demand site-specific delivery/release of therapeutics to prevent CNS diseases. His main research interest is developing nanomedicine along with exploring novel methods of brain delivery and wearable sensors for personalized health care. Dr. Kaushik received his Ph.D. (Chemistry and Biosensors) in collaboration with the National Physical Laboratory and Jamia Milia Islamia, New Delhi, India.

Abstract:

Advancements in nano-bio-technology revolutionized health care management via investigating nanomedicine and delivery for targeted diseases. Due to easy tunable performance features these systems serves in a controlled manner and significantly useful for personalized health care. Keeping this in view, magnetically-guided brain delivery of magneto-electro nanoparticles (MENPs ± 25 nm), a drug nano-carriers capable to exhibit a. c. magnetic field stimuli responsive on-demand drug release, in C57Bl/J mice has been demonstrated. Our findings confirm uniform distribution of MENPs in the brain of mice without causing clinical toxicity and altering neurologic behavior. However, the translation of this brain delivery method for humans is not yet developed due to a mismatch of available static magnet dimension in relation to the human brain size and shape. Aiming to develop personalized nanomedicine to eradicate neuro-HIV/AIDS, we demonstrated magnetically-guided brain delivery of MENPs to the brain of an adult female baboon (Papio hamadryas) using magnetic resonance imaging (MRI) as a navigation tool. An optimized dose of MENP (22 mg/13 kg) suspended in 100 mL PBS was injected into the baboon vasculature via the saphenous vein with a flow rate of 220 mL/hr. After injection, the baboon was placed under static MRI magnetic exposure for 3 hours to achieve magnetically-guided brain delivery. MRI image analysis confirmed MENPs distribution within the brain regions such as basal ganglia, hemisphere, and vertex. The results of histopathology and blood toxicity profile studies confirmed that injected MENPs did not cause any toxicity or metabolic abnormalities. We propose utilizing MRI as a potential navigation tool for the brain delivery of magnetic therapeutic formulations to treat brain diseases for personalized health care.

  • Personalized Nanomedicine
Location: Hilton Tokyo Narita