Michal M. Godlewski,
Warsaw University of Life Sciences WULS-SGGW, Poland
Title: New biocompatible oxide nanoparticles as carriers of bioactive compounds through the blood-brain barrier
Biography
Biography: Michal M. Godlewski,
Abstract
Blood-brain barrier is major obstacle for drug delivery to the brain. In this study we focused on oxide nanoparticles (NPs) as potential drug carriers. Mice received suspension of Y2O3:Tb:Lectin NPs (10mg/ml; 0.3ml/mouse) via gastric gavage (IG) and were sacrificed after 24h, 48h and 1 week. Control group received equivalent suspension of pure lectin. All protocols were conducted according to EU guidelines and approved by LEC agreement No 44/2012. Following the sacrifice, brain tissue was collected for the analyses under confocal microscope and scanning cytometry. Lectins were chosen as a perfect model substance for the use of NPs as carriers, due to the fact that physiologically they are not absorbed from the gastrointestinal tract.
Control group exhibited extremely low signal for lectin not exceeding background level. In the group which received Y2O3:Tb:Lectin, signal for lectin coincided with NPs red fluorescence in the brain as soon as 24h after IG. Following 48h, the convergence lowered and after 1 week only free lectin were observed in the brain tissue.
In conclusion, oxide NPs proved able to transport bioactive compounds through the blood-brain barrier. After entering brain tissue complexes of nanoparticles and lectin dissolved and free lectin was deposited in the tissue.