Day 1 :
Keynote Forum
P R Raghavan
Nanorx Inc., USA
Keynote: Nurture though nature: The story of metadichol: A unique nano lipid
Time : 08:40-09:20
Biography:
P R Raghavan is a CEO of Nanorx Inc. and has a PhD in Organic Chemistry from Oregon State University (1979) and an MS in Chemistry (1972) from IIT, Mumbai, India. He has worked on drug discovery for over 25 years at Columbia University, Max-Planck Institute, Germany, Ciba-Geigy (now Novartis) and Boehringer Ingelheim. He has over 15 US and international patents and another 15 pending patent applications
Abstract:
Metadichol (US patent 8,722,093 and 9043,383) is a nano emulsion of long-chain alcohols found in many foods that is present in foods such as rice, sugar cane, wheat and peanuts. Metadichol acts as inverse/Protean agonist on Nuclear Vitamin D receptors (VDR) (US patents 9,006,292, 8,722.093, 9.034, 383) that are present in cells throughout the body to stimulate the immune system. Metadichol® is first of a class of unique nano emulsion molecules that are active against viruses, bacteria, and parasites. In addition to acting on VDR, it shows cross reactivity against other nuclear receptors and how this leads to mitigation of various chronic diseases like diabetes, hypertension etc. Gene expression analysis and human clinical case studies will be presented. Because, it consists of natural components of conventional foods and has no known negative side effects, Metadichol has the potential to serve as a novel, broad-spectrum treatment for many diseases that confront public health today bringing the concept of health care for all at a price we can afford
Keynote Forum
Thomas J Webster
Northeastern University, USA
Keynote: Two decades of commercializing nanomedicine to improve disease prevention, detection and treatment
Time : 09:20-10:00
Biography:
Thomas J Webster is the Chemical Engineering Art Zafiropoulo Chair at Northeastern University. His lab group published 9 textbooks, 48 book chapters, 403 articles, and 32 provisional/full patents. He has received numerous honors: 2012, Fellow, American Institute for Medical and Biological Engineering; 2013, Fellow, Biomedical Engineering Society; 2015, Wenzhou 580 Award; 2015, Zhejiang 1000 Talent Program; 2016, IMRC Chinese Academy of Science Lee-Hsun Lecture Award; 2016, Fellow, Biomaterials Science and Engineering; and 2016, Acta Biomaterialia Silver Award. He has regularly appeared on BBC, NBC, ABC, Fox, National Geographic, and many other news outlets. 144 students have graduated under him
Abstract:
Objective: Nanotechnology (or the use of materials with one dimension less than 100nm) has been revolutionizing the field of medicine for several decades due to the ability of nanomaterials to mimic natural features of healthy tissues. However, issues remain such as toxicity, assembling nanomaterials into functional organs, efficacy, drug loading, cost and lengthy FDA approval times have still proven to be significant obstacles. The objective of this talk is to summarize recent advances in developing nanostructured artificial organs for quick regulatory approval.
Methods: Approaches such as top-down and bottom-up nano approaches along with 3D printing, cast-molding, and other techniques to create artificial organs will be covered. Approaches have been shown to be versatile using ceramics, metals, polymers and composites thereof. In vitro and in vivo studies will be covered. Nanoparticle synthesis will also be covered with challenges and promises.
Results: Such approaches have led to improved interactions with mammalian cells (such as bone, cartilage, vascular, neural, bladder, etc.,) and decreased interactions with immune cells (such as monocytes, macrophages, etc.,) to regenerate organs. Recently, results have shown the ability to decrease bacteria functions without using antibiotics. Lastly, a new approach to medicine focused on controlling Pico scale events will also be introduced where one can dictate electron interactions within a material to improve cellular functions leading to greater organ regeneration.
Conclusions: In summary, this talk will cover what has been learned over the past several decades of translating nanotechnology to improve organ replacement while emphasizing future developments that we should expect for the field to grow (such as Picotechnology).
Keynote Forum
Chad Schwartz
Beckman Coulter Life Sciences, USA
Keynote: Advancing science through discovery (Exhibitor)
Time : 10:20-10:35
Biography:
Abstract:
Keynote Forum
Oara Neumann
Rice University, USA
Keynote: Enhanced T1 MRI contrast and fluorescence stability within a plasmonic core-shell nanoparticle
Time : 10:35-11:15
Biography:
Oara Neumann has completed her PhD and Post-doctoral study in Applied Physics at Rice University and MS from Weizmann Institute of Science, Israel, and Bucharest University, Romania. She is a Research Scientist in Naomi Halas group at Rice University. She holds 12 patents and has published more than 25 papers in reputed journals.
Abstract:
Multifunctional plasmonic nanostructures have enormous potential in the treatment of solid tumors; however, tracking particles with drug cargo and triggering the release of the cargo in mapped tumors is still impossible. To overcome this challenge, we have developed an MRI and fluorescent active nanostructure nanomatryoshka. This new nanostructure with IR plasmonic signatures is composed of a 50nm Au core surrounded by dye molecules and Gd(III)-DOTA chelate doped SiO2 inner-shell and an outer Au shell. The experimental results demonstrate an enhanced T1 relaxation (r1 ~ 24mM-1s-1 at 4.7 T) compared to the clinical Gd(III)-DOTA chelating agents (r1 ~ 4mM-1s-1). Further, this design preserves the fluorescence signal (65%) after 24 hours of exposure, leading to enhanced fluorescence photostability (23x). This dual-imaging functionality nanosystem increases MRI sensitivity by concentrating Gd(III) ions into the Gd-NMs, reduces the potential toxicity of Gd(III) ions and dye molecules by preventing their release in vivo through the outer Au shell protection, and the terminal gold layer surface can then be functionalized to increase cellular uptake, circulation time, or thermal drug-release properties.
Keynote Forum
Yashwant Pathak
University of South Florida, USA
Keynote: Systemic biology approaches to treat age related macular degeneration using nano-particulate drug delivery systems: A review
Time : 11:15-11:55
Biography:
Yashwant Pathak has completed his PhD in Pharmaceutical Technology from Nagpur University, India and EMBA and MS in Conflict Management from Sullivan University. He is a Professor and Associate Dean for Faculty Affairs at College of Pharmacy, University of South Florida, Tampa, Florida. He has extensive experience in academia as well as industry, has more than 150 publications and two patents and two patent applications, 16 books including five books in Nanotechnology and five in Nutraceuticals and Drug Delivery Systems
Abstract:
Age-related macular degeneration (AMD) is the leading cause of irreversible blindness in people 50 years of age or older in the developed world. More than eight million Americans have age-related macular degeneration, and the overall prevalence of advanced age-related macular degeneration is projected to increase by more than 50% by the year 2020. Recent advances in clinical research helps in better understanding of the genetics and pathophysiology of AMD. The systemic biology information which has revealed several mechanisms causing the AMD which can be used for developing new therapies designed to prevent and help treat it the AMD. With the advances in nanotechnology, characterization techniques of nanoparticles and the enormous surface nanoparticles provide if used as a carrier for drug, researches in the ophthalmic drug delivery are gearing towards using these NPDDS (Nano-particulate drug delivery systems) for treating AMD. We have used thermo reversible polymeric gel systems to deliver various drugs which hold promises to treat mitigate and prevent AMD. The review will cover various strategies based on systemic biology of AMD and our efforts to deliver the appropriate drugs to treat various symptoms of AMD using NPDDS.
Keynote Forum
Jordi Arbiol
Catalan Institute of Nanoscience and Nanotechnology, Spain
Keynote: Free-standing nanostructures at atomic scale: From growth mechanisms to local properties at the nanoscale
Time : 11:55-12:35
Biography:
Jordi Arbiol has completed his graduation in Physics at Universitat de Barcelona (UB) in 1997, where he also obtained his PhD (European Doctorate and PhD Extraordinary Award) in 2001. He was an Assistant Professor at UB. From 2009 to 2015, he was the Group Leader at Institut de Ciència de Materials de Barcelona,
ICMAB-CSIC. Since 2017, he has been the President of Spanish Microscopy Society (SME). Since 2015, he has become the leader of the Group of Advanced Electron Nanoscopy at Institut Català de Nanociència i Nanotecnologia (ICN2). He has been awarded with the EU40 Materials Prize 2014 (E-MRS) 2014 EMS Outstanding Paper Award and the PhD Extraordinary Award in 2001.
Abstract:
Technology at the nanoscale has become one of the main challenges in science as new physical effects appear and can be modulated at will. Superconductors, materials for spintronics, electronics, optoelectronics, sensing, energy applications and new generations of functionalized materials are taking advantage of the low dimensionality, improving their properties and opening a new range of applications. As developments in materials science are pushing to the size limits of physics and chemistry, there is a critical need for understanding the origin of these unique physical properties (optical and electronic) and relate them to the changes originated at the atomic scale, e.g., linked to changes in (electronic) structure of the material. In the present work, I will show how combining advanced electron microscopy imaging with electron spectroscopy, as well as cathodoluminescence in an aberration corrected STEM will allow us to probe the elemental composition and electronic structure simultaneously with the optical properties in unprecedented spatial detail. The talk will focus on several examples in advanced nanomaterials for optical, electronics and energy applications. In this way, the latest results obtained by my group on direct visualizing and modeling materials at atomic scale will help to understand their growth mechanisms (sometimes complex) and correlate their physical and chemical properties at sub-nanometer with their atomic scale structure. The examples will cover a wide range of nanomaterials: quantum structures self-assembled in a nanowire: quantum wires (1D) and quantum dots (0D) and other complex nanowire-like morphologies for photonic and energy applications (LEDs, lasers, quantum computing, single photon emitters, water splitting cells and batteries).
Keynote Forum
Eleonora Babayants
Galaxy Consulting, USA
Keynote: GxP/GMP and its consequences for documentation and information technology systems
Time : 13:30-14:30
Biography:
Eleonora Babayants is the Founder and President of Galaxy Consulting. She is a Documentation Management Professional and hands-on consultant with over 25 years of experience in Documentation and Records Management, Document Control, Regulatory Compliance, Internal and External Auditing, Electronic Document Management Systems, Information Governance, and Change Management. Her past work includes Development and Implementation Regulatory Compliance Processes and Procedures, Leading Implementation and Administration of Document Control Systems in full Compliance with Regulatory Requirements, Enabling
Enterprise Search, Improving Systems Information Architecture, Creating and Implementing Users Training Programs. She led Electronic Document Management Systems Selection and Deployment, administered and supported these systems, Web Information Portals, Knowledgebase Applications, Recommended and Implemented Re-Structuring of the content and the Information Architecture of these systems. She has worked very closely with IT to do feasibility assessment and to capture users’ requirements. Her experience spans multiple industries including Biomedical, Pharmaceutical, and Medical Devices Companies.
Abstract:
Documentation is a critical tool for ensuring GxP/GMP compliance. This is what GMP states about document control: Each manufacturer shall establish and maintain procedures to control all documents that are required. In the regulated environment which must be GxP/GMP compliant, document control is the cornerstone of the quality system. It is so important that if an external audit identifies deficiencies in the document control system, the entire organization can be shut down. There are also GMP requirements for information technology. For a drug to be produced in a GxP/GMP compliant manner, some
specific information technology practices must be followed. Computer systems involved in the development, manufacture, and
sale of regulated product must meet certain requirements. Change control within quality management systems (QMS) and information technology (IT) systems is a formal process used to ensure that changes to a product or system are introduced in a controlled and coordinated manner. In the regulated industries, manufactures are required to use a change control procedure. In this presentation, I will discuss the connection between GxP/GMP and document control. I will describe details of document control procedures and the role of Quality Assurance in the documentation systems. I will review GMP requirements for information technology and how computer systems including documentation management systems must meet GxP/GMP requirements. I will also review change control procedure and how it should be used in GxP/GMP environment.
Keynote Forum
Branislav Vlahovic
NASA University Research Center, USAin
Keynote: Highly selective and sensitive biochemical detector for medical applications
Time : 14:30-15:10
Biography:
Branislav Vlahovic is the Director of the National Science Foundation Computational Center of Research Excellence, NASA University Research Center for Aerospace Device and NSF Center Partnership for Research and Education in Materials at North Carolina Central University. In 2004, he was awarded by the Board of Governors of the University of North Carolina Oliver Max Gardner statewide award for his research and contribution to science. He has published more than 300 papers in peer reviewed journals on: Nanotechnology, Nanostructures, Tunneling and Charge Transfer between Nanostructures, Pulsed Laser Deposition, Nonlinear Optics, Detectors and Devices, Nano Photonics, Semiconductor Structures, Photovoltaic, and Genomics.
Abstract:
We consider that novel biochemical sensor based on the charge transfer between detector’s semiconductor nanostructures and analyte molecules that will be detected. It is an original concept that relies on the tunneling between an analyte molecule and the discrete energy levels of the detector’s nanostructures. The energy levels in nanostructures, which depend on quantum confinement and external effects, are tailored to mimic the energy levels of the analyte to be detected. Charge tunneling between the detector’s nanostructures and the analyte will occur only if the analyte has the energy levels matching the energy levels of the detector’s nanostructures. This completely new concept for biochemical sensor, which leverages the unique properties of nanostructured materials and design and optimization of detector’s nanostructure’s, provides label free detection and identification of a wide range of analyses, with single molecule sensitivities. Such unique detection method allows for continuous, instantaneous real time, high selectivity, high sensitivity, miniature in situ characterization device that could be implemented for various medical applications. The sensor may be a standalone unit, or it can serve as a part of an instrument, enhancing selectivity and sensitivity of that device. Discussed will be modeling of the optoelectronic properties of nanoscale materials and QDs for realistic QD detector ensembles, including the study of collective effects on energy level spectra and charge transfer processes between nanostructures; its application for detection in fluids; and the production of the sensor components and the final prototype device in our nanotechnology laboratory
Keynote Forum
Alberto Coelho
University of Santiago de Compostela, Spain
Keynote: 3D Printing in chemistry: Development of monolithic heterogeneous 3D-catalysts for solution phase chemistry in drug discovery
Time : 15:10-15:50
Biography:
Alberto Coelho has completed his PhD in Pharmacy from Universidade de Santiago de Compostela (2003, Spain) and Post-doctoral studies in Europe (Belgium, Italy and Portugal). He is a Member of the Instituto de Cerámica de Galicia (Spain). He has published more than 50 papers in reputed journals and has been serving as an Editorial Board Member of repute. His expertise is focused on Medicinal Chemistry, Drug Discovery, Catalysis and Nanomaterials
Abstract:
The application of efficient catalytic processes instead of stoichiometric ones is a main requirement for a sustainable and environmentally friendly chemistry. Developing hybrid heterogeneous catalysts with multiple active sites, as well as designing sequential reactions using multiple catalysts in the same reaction vessel with eco-friendly conditions are ambitious challenges to achieve the objectives mentioned above. Shape and size are key aspects in the design of heterogeneous catalysts. However, integration of micro and macroscopic properties in the design of new devices and three-dimensional shape defined catalysts is an aspect barely explored so far. 3D printing technology offers the key advantage of the fabrication of threedimensional physical objects from a digital model, taking a virtual design from computer aid design (CAD) software and its reproduction layer by layer until the physical definition of the layers gives the designed product. In addition, 3D printing can make a significant impact in the field of the chemical synthesis and catalyst manufacturing, in particular because the easy and economy which 3D-printers currently available permits a broad spectrum of materials to be printed, including solutions of comparatively low viscosity. The application of 3D-printed devices, appropriately modified on the surface, to perform complex catalytic processes such as multicatalytic multicomponent reactions (MMCRs) is a field nothing explored at all. In this work, we address the different chemical strategies for the appropriate functionalization of ceramic materials obtained by 3D-printing, as well as the combined application of these obtained 3D-monolithic catalysts, reacting cooperatively, in the development of new designed MMCRs.
Keynote Forum
Galaxy Consulting, USA
Galaxy Consulting
Keynote: GxP/GMP and its consequences for documentation and information technology systems
Biography:
Abstract:
- Bionanotechnology | Nanoscale materials | Nanocomposites | Nanoparticles and Nanomedicine | Emerging Nanomedicine | Computational Studies in Nanoparticles | Research and Development of Nanomedicine
Location: las vegas, USA
Chair
Istvan Toth
The University of Queensland, Australia
Co-Chair
Jordi Arbiol
Catalan Institute of Nanoscience and Nanotechnology, Spain Session
Session Introduction
Long-ping Wen
South China University of Technology, China
Title: Exploiting nanomaterial-induced pro-survival autophagy for cancer therapy
Time : 16:10-16:30
Biography:
Long-ping Wen has graduated from Xiamen University in 1982 (BS) and obtained his PhD from University of California, Los Angeles, USA in 1988. He has over 30 years of experience in Biomedical Research at various Academic Institutions in the USA, Singapore and China. He is currently a Full Professor at South China University of Technology, with a research interest focusing on Nanobiology and Nanomedicine. He has published over 100 papers, including 51 corresponding author papers during the past 10 years, in the various Sci journals such as Nature Biotechnology and Nature Materials
Abstract:
Autophagy, a key cellular degradation process, is a common response of cells upon exposure to nanomaterials. A variety of nanomaterials, including carbon, metal, and rare earth oxide nanoparticles, have been demonstrated to induce elevated level of autophagy in different cell types. Autophagy induced by nanomaterials may be either pro-survival or pro-death, and the different fate on the affected cell can be differentially exploited to enhance cancer therapy. In this talk, I will focus on the pro-survival aspect of the autophagy induced by namomaterials and how it may be exploited for cancer therapy. First, I will summarize our published work on silver nanoparticle-induced autophagy, its pro-survival nature and the enhanced therapeutic efficacy upon its inhibition. Then I will present our recent unpublished work on cancer photothermal therapy (PTT) facilitated by a type of CuPd tetrapod nanoparticles (CuPd TNPs-1). These unique nanoparticles exhibited superior NIR-assisted photothermal conversion efficiency and induced pro-survival autophagy in a shape- and composition-dependent manner. Inhibition of autophagy with the autophagy inhibitor 3-methyl adenine (3-MA) had a remarkable synergistic effect on the anti-cancer efficacy of CuPd TNPs-1-mediated PTT both in triple-negative (4T1) and drug-resistant (MCF7/MDR) breast cancer models, as it helped to achieve a level of efficacy unattainable with CuPd TNPs-2, the similarly shaped alloy nanoparticle that had a higher photothermal conversion efficiency but no autophagy-inducing activity. This work provided a proof-ofconcept for a novel chemo-PTT strategy, in which traditional chemotherapeutic agents are replaced by autophagy inhibitors. This strategy is applicable to any PTT-ready nanomaterial with the capability of inducing pro-survival autophagy and should be particularly useful for eradicating drug-resistant cancer.
Daniel Chojnowski
Howard & Howard Attorneys PLLC, USA
Title: The never-ending internal struggle: Do I disclose my invention and attempt to obtain a patent or forgo a patent and keep it as a trade secret?
Time : 16:30-16:50
Biography:
Daniel Chojnowski is an Intellectual Property Attorney who concentrates his practice in the chemical and material science arts. His practice includes global patent procurement and opinion preparation. He also counsels clients on clearance/freedom-to-practice issues, as well as on strategies for management of their global intellectual property portfolios. Prior to becoming an attorney, he was employed as an RD&I Chemist
Abstract:
Companies are often forced to internally debate whether to apply for a patent or keep the invention “secret.” This question is particularly ripe in the field of nanotechnology, where methods of producing nano particles/structures are difficult to reservably engineer. Thus, the consequences of the public disclosure mandated during the patenting process can be especially harsh if an attempt to obtain a patent is ultimately unsuccessful. This presentation will explore the essential factors that should be balanced when debating whether to apply for a patent or maintain a trade secret. This presentation will also discuss relatively new procedures at the United States Patent and Trademark Office that, if leveraged correctly, will allow an applicant to have its cake and eat it too.
Shuhua Bai
Husson University, USA
Title: Exosome nanovesicles for the homing delivery of therapeutics into autologous cancer cells
Time : 16:50-17:10
Biography:
Shuhua Bai has obtained his PhD from the School of Pharmacy, Texas Tech University Health Sciences Center. He is an Associate Professor in the School of Pharmacy, Husson University. He has had more than 16-years of research experience on the Nanoparticle-based drug delivery and development. He has published more than 30 research papers in reputed journals. He recently received the 2017 Pharmaceutical Research Meritorious Manuscript Award, presented by the American Association of Pharmaceutical Scientists (AAPS)
Abstract:
Extracellular vesicles (EVs) are naturally occurring membrane particles that mediate intercellular communication by delivering molecular information between cells. In this study, we propose to package therapeutics agents in endogenous nanovesicles, known as exosomes, derived from cancer cells. It is expected that autologous cancer cell-derived exosomes can be taken by cancer cells themselves via homing selectivity and bring the entire payload to the autologous tumor cells. Exosomes were isolated from human adenocarcinoma A549 and brain endothelial bEND.3 cell cultures using centrifugation. They presented nanosized vesicles measured by a nanosizing system. Fluorescent rhodamine 123 and anticancer drugs including paclitaxel and doxorubicin were incorporated into exosome nanoparticles by diffusion. Designed siRNA to inhibit Intercellular Adhesion Molecule-1 (ICAM-1) was loaded into exosome with the assistance of transfection reagent. Fluorescence intensity in the cells treated with autologous cell-derived exosome delivered markers was significantly increased. Exosome-delivered anticancer drugs significantly decreased cell viability in autologous cells compared to other exosomes. Exosome-delivered siRNAs significantly enhanced the knockdown efficacy compared to siRNA alone, dependent on exosome original sources and loading methods. ICAM-1 siRNA formulated in autologous lung cell-derived exosomes with the assistance of transfection agent showed the best inhibitory effect on the ICAM-1 expression compared to other treatments. Cancer cell-derived exosomes could be used as effective carriers, bringing therapeutic agents into the cells and increasing efficacy to their parental cells. The use of cancer cell-derived exosomes will be further investigated for individualized and targeted lung cancer therapy
Amparo Verdu Solis
Bioinica, Spain
Title: Optinanopro: Novel nanocomposites and nanocoatings and their applications in the packaging, automotive and solar panel processing lines
Time : 17:10-17:30
Biography:
Amparo Verdu Solis holds a University Degree in Industrial Chemistry by the Polytechnic University of Valencia and Master’s in Plastics and Rubber Materials. For 10 years, she developed her career as Researcher in AIMPLAS, Plastics Technology Centre. Currently, she is the R&D Project Manager of Bioinicia, SL, experts
in Nanotechnology and Electro-Hydro Dynamic Processing. She has collaborated as an Expert Member in the European Committee for Standardization CEN/TC 249/WG 7/TG one Biodegradable mulch films. Now, she is involved in SBIOC Spanish BioCluster as President of the Cluster.
Abstract:
Nanocomposites have special physical and chemical properties and an array of potential applications, in particular they can be used to adapt surface properties and introduce additional functionalities. Performance of nanocomposites depends on a number of parameters, but nanoparticles dispersion and distribution state remain the key challenge in order to obtain the full nanocomposites’ potential. Optinanopro project has worked to demonstrate the benefits of the introduction of nanotechnology into packaging, automotive and photovoltaic materials production lines. Special mention to the development
and industrial integration of electrospray nano-deposition, online dispersion and monitoring system to ensure a constant quality of produced nanocomposites. Nano-enhanced coating has been applied by electrospinning, a phenomenon that occurs
when an appropriate electric field is applied to a conductive solution. Electrospinning technology has been used to obtain both self-cleaning OPVs and product repellent surfaces (with tailored repellence to selected liquids) for easy emptying packaging. Bioinicia has achieved materials with tailored polarity including super hydrophobic properties but also amphiphobic ones, a quite peculiar behavior that is much less reported in the literature. In the case of OPV surfaces, hydrophobicity will allow the panels to self-clean from dirty rain, whereas the same effect will allow facilitating the emptying and therefore reduce leftover at end of life in case of polar liquids/pastes being packaged (e.g. oil in water emulsions for cosmetics). The process required a novel multistep approach that has been patented by Bioinicia on how to use electro-hydrodynamic processes for obtaining stable layers with target characteristics.
Sallahuddin Panhwar
University of Engineering & Technology, Pakistan
Title: Amparo Verdu Solis holds a University Degree in Industrial Chemistry by the Polytechnic University of Valencia and Master’s in Plastics and Rubber Materials. For 10 years, she developed her career as Researcher in AIMPLAS, Plastics Technology Centre. Currently, she is the R&D Project Manager of Bioinicia, SL, experts in Nanotechnology and Electro-Hydro Dynamic Processing. She has collaborated as an Expert Member in the European Committee for Standardization CEN/TC 249/WG 7/TG one Biodegradable mulch films. Now, she is involved in SBIOC Spanish BioCluster as President of the Cluster.
Time : 17:30-17:50
Biography:
Sallahuddin Panhwar is a PhD Student of US-Pakistan Center for Advanced Studies in Water (USPCAS-W) at Mehran Univeristy of Enineering & Technology Jamshoro, Pakistan. Currently he is exchnage visiting scholar at the Univerosty of Utah, U.S.A. He has published more than 8 research papers in reputed journals and attended national and international conferences.
Abstract:
The aim of this study was to develop a simple electrochemical sensor for detection of (E.coli 25922) from water using gold nanoparticles. The water security and microbiological defense applications are globally concerns because of accuracy in the results and time saving technology. The traditional detection method of bacteria is requiring more time for the results. The biosensor reduce the detection time from 2 to 3 days to less than one hour with a simple identification method. The detection of pathogenic bacteria (E.coli 25922) is pivotal to public health for the water and food security. The electrochemical detection is applied for the detection of E. coli. However, in resulting the lowest bacterial concentration was weekly at 1 x 101 CFU/ml and the strongly on 1 x 106 CFU/ml. The electrochemical signal was increased with the increasing concentration of E. Cali. These results confirmed that the AuNPs-GCE is an effective approach to highly sensitive detection for the E.coli.
Shuhua Bai
Husson University, USA
Title: Exosome Nanovesicles for the Homing Delivery of Therapeutics into autologous cancer cells
Biography:
Abstract:
- Nanoscale materials | Sustainable (or) Green Nanotechnology | Nanofabrication | Nanocomposites| Smart nanotechnology | Toxicology of Nanoparticles |Research and Development of Nano Medicine | Polymer Nanoparticles and Nanomedicine | NanoParticles and Nanomedicine
Location: Las Vegas, USA
Chair
Xudong Huang
Harvard Medical School, USA
Co-Chair
Toyoko Imae
National Taiwan University of Science and Technology, Taiwan Session Introduction
Session Introduction
Nicky Thomas
University of South Australia, Australia
Title: A Trojan Horse strategy for the delivery of biologicals
Time : 14:15-14:40
Biography:
Nicky Thomas is an NHMRC Research Fellow at the School of Pharmacy and Medical Sciences, University of South Australia and is working in the Professor Clive Prestige’s group. He is a trained Pharmacist with several years of experience in both hospital and community pharmacies. In 2012, he has been awarded his PhD in Pharmaceutical Sciences from the University of Otago, New Zealand. His PhD research was concerned with the development and in vitro and in vivo characterization of nano emulsions for drug delivery. He has joined UniSA in 2012 to work on novel treatments against bacterial biofilms. Building on his expertise in nanomedicine-based drug delivery systems he has been awarded an NHMRC Early Career Researcher Fellowship in 2014 for the investigation on the interaction of antimicrobial therapies with bacterial biofilms. In 2017, he established the Adelaide Biofilm Test Facility at UniSA’s Sansom Institute for Health Research, SA’s first facility dedicated to test antimicrobials and pharmaceutical products in a range of in vitro and in vivo biofilm models. His main area of research is anti-infective treatment options against biofilms; oral drug delivery and lipid-based drug delivery
Abstract:
Background: Glycoside hydrolases have emerged as potent, novel therapeutics that can disrupt biofilms, thereby increasing the susceptibility of the residing bacteria to co-administered antibiotics. The broader clinical use of glycoside hydrolases such as alginate lyase (AL) is limited due to challenges in maintaining enzyme stability, adequate delivery and release of the enzyme at the site of infection. Herein, we present a Trojan horse carrier for AL using environment-sensing lyotropic liquid crystalline gels (LLC).
Aim: The aim of this study was to design a LLC-gel carrier based on the lipid glycerol monooleate to protect, deliver and release
AL in combination with the antibiotic gentamicin (GENT) as a novel anti-biofilm strategy.
Methods: The effect of Pseudomonas lipase on the release of AL/GENT from LLC-gels was evaluated and the efficacy of the gel was determined over 1 week in vitro against biofilms formed by alginate producing P. aeruginosa (clinical isolate) and compared to an unformulated simple drug solution. Finally, the stability of AL after fabrication of the LLC gel was assessed.
Results: GENT and AL were released at different rates and extent from the LLC-gels (10% AL over nine days; 60% GENT over two days, respectively). Addition of Pseudomonas lipase increased AL release >2-fold (20-30% within two days). The LLCgel demonstrated similar anti-biofilm activity (2.5 log reduction in CFU) compared to unformulated solution, confirming preservation of AL activity in the LLC-gels. Interestingly the antimicrobial effect could not be sustained over extended period (>2 days) which was attributed to a gradual loss of AL activity from prolonged exposure to 37oC during the assay, rather than short exposure to higher temperatures (60oC) during LLC-gel fabrication
Muhammad Yasir Ali,
Philipps University Marburg, Germany
Title: Aptamer conjugated nanoparticles as carrier for targeted anticancerous drug
Time : 14:40-15:05
Biography:
Muhammad Yasir Ali has done masters in Pharmaceutics from The Islamia University of Bahawalpur, Pakistan and was working in GC University Faisalabad, Pakistan in Faculty of Pharmaceutical Sciences. Currently, he is doing his PhD at the Philipps University Marburg, Germany
Abstract:
Targeting ErbB receptors has become a popular tool for drug delivery to overcome the downstream cytoplasmic signaling mechanisms. The cascade of these reactions, RAS-MAPK and PI3K-Akt are responsible for cell proliferation and antiapoptotic ways of cell growth and resistance to already approved antibody and drug therapies against these receptors. Among other approaches of cell targeting, use of aptamers has become one of the major choices because of their selective targeting and low immunogenicity. Aptamer can easily be synthesized chemically. The current project involved the use of Sorafenib loaded biodegradable PLGA nanoparticles prepared by solvent evaporation method. The surface of these particles was coupled with aptamer (aH3) against ErbB3 using EDC/NHS. For physico-chemical characterization, particls size, zeta potential and shape was determined before and after the surface modification. Antiproliferation assay was conducted using ErbB3 positive MDAMB- 231 and ErbB3 negative SKOV-3 cell lines. Cell migration and displacement was checked by scratch test and agarose gel method, using EGFR and NRG1 as positive control. Cellular uptake studies were evaluated using confocal laser scanning microscopy (CLSM). A decrease in cell viability was observed after incubation with aptamer modified nanoparticles in case of MDA-MB-231 cells. The opposite was observed in SK-OV-3 cells due to he absence of ErbB3 receptors. Scratch tests performed with modified nanopartciles revealed a change in the migration pattern of the cells depending upon the presence of absence of the ErbB3 receptors. CLSM results showed the receptor mediated intracellular uptake of nanoparticles. It was thus obvious from the results that the specificity of aptamer can be used for targeted drug delivery.
Arend Laurence Mapanawang
Yayasan Medika Mandiri Foundation, Indonesia
Title: Chemical bonding characters of love herbal medicine
Time : 15:05-15:30
Biography:
Arend L Mapanawang has completed his PhD from Department of Pharmacy, College of Health Sciences, Yayasan Medika Mandiri Foundation, Halmahera, Indonesia. He is the Director of College of Health Sciences (STIKES Halmahera), Medika Mandiri Foundation, Halmahera, Indonesia. He is the Head of Internal Medicine Departement of Bethesda Hospital in Tobelo North Halmahera, North Moluccas.
Abstract:
Anti-oxidant with a very high concentration of IC50=6.54ng/ml extracted from Golobe halmahera (Zingiberaceae) fruit had made this local fruit a very good candidate for improving human health. Based on this finding, a medicine named as love herbal had been fabricated with a multitasking healing in human body problems. In order to analyze the physical working system of this medicine, one investigated the optical properties of it by using chemical bonding spectroscopy method. We obtain that such medicine has optical uniqueness behaviors which might be closely associated with multitasking healing of human body problems, for example in improving antibody protection of human body from cancer diseases as well as the health of heart and liver. The main indicator associated with that was from a small C-F bonding peak at 1360cm-1 contributed by love herbal related to the C-H bonding. Moreover, the presence of love herbal in tap water mixed with betadine created a higher peak of C C as well as the shift of C-H bonding from 2949cm-1 to be 3013cm-1. While love herbal interactions with betadine in tap water made a little decrease of C=C bonding at 1697cm-1. We suggest that this interesting love herbal may be one of the best multitasking healing medicine.
Imran Tariq
Philipps University of Marburg, Germany
Title: Enhanced gene delivery by lipid triblock nanocarriers
Time : 15:30-15:55
Biography:
Imran Tariq is working as an Assistant Professor at University of Punjab, Lahore, Pakistan. He has completed his Master’s in Pharmaceutics from University of the Punjab. Currently, he is pursuing his PhD at the Philipps University of Marburg, Germany. He has published more than 25 research papers in well reputed journals and has served as a Member in Scientific Editorial Board of reputable journals
Abstract:
Non-viral vectors system is the prerequisite for the efficient gene delivery with minimal toxicity, against many incurable diseases. Polyamidoamine (PAMAM) dendrimers with a positive charge has a great tendency to complex nucleic acid (dendriplexes) like plasmid DNA (pDNA) and small interfering RNA (siRNA). They shield them from enzymatic degradation, thus facilitate endocytosis and endosomal release. The current study was aimed to develop a delivery system by using lipid modified nanocarrier to enhance gene delivery and knock downing of targeted gene. These liposomal encapsulated dendriplex system can further reduce the unwanted cytotoxicity of this cationic system, which enhance cellular uptake of nucleic acid. Broad range of lipid combination were used to optimize the lipodendriplexes and tested for their physicochemical characterization including size, shape and zeta potential. The optimized lipodendriplexes were tested for anti-proliferation, pDNA transfection, cellular uptake, siRNA knock downing and heparin stability. The optimized system has shown significant improvement in cytotoxicity (p<0.05), pDNA transfection (p<0.01), with higher cellular uptake and gene knockdown efficiency, as compared to parent dendriplex system. The hemocompatibility intergrity in the presence of natural polyanion support the behavior of optimized lipodendriplexes system in biological environment. Therefore, further in vivo studies can be a done to explore the full potential of this platform, using ligand specific targeted system, against different cancer and genetic disorders.
Hendry Izaac Elim
Pattimura University, Indonesia
Title: Electronics physical system of large antioxidant structure in herbal medicine based zingiberaceae fruit: Understanding and application
Time : 16:15-16:40
Biography:
Hendry Izaac Elim, PhD, is a young Scientist of Pattimura University. Currently, he is an experienced Indonesian Scientist of Nanoscience and Nanotechnology, and also Head of the Nanotechnology Research Center and Innovative Creation. He is the Chairman for Nanomaterials for Photonics Nanotechnology Laboratory (N4PN Lab), Physics Department, Faculty of Mathematics and Natural Sciences (FMIPA UNPATTI), Ambon, Indonesia, and regular Member of the Indonesia Theoretical Physicist. After his PhD at NUS, he worked as a Post-doctoral fellow in Physics Department of NUS. He published over 41 papers with h-index of 23, and citation more than 2000 which made him elected as one of Pattimura University Young Scientists in 2017 awarded for the first time since 1963 by Prof. M.J. Saptenno.
Abstract:
In this present study, we introduce an electronic physical system of a large antioxidant chemical structure based on herbal medicine fabricated using Zingiberaceae fruit (Maluku Golobe) consisted of many different varieties mainly such as Halmahera rambutan Golobe, Halmahera original Golobe, and Ambon durian Golobe. A simple electronics circuit model as a prototype of nanoscale antioxidant structure with the size of 15 carbon atoms plus 2 oxygen atoms was employed to understand the highest antioxidant herbal fruit associated with highly carbon-carbon bonding and carbon-oxygen bonding as well as its interaction with water, the remarkable substance in human body of the immune system. Such electronic physical system in herbal medicine is discussed with their prominent applications particularly as HIV herbal medicine. A brief discussion on how the large antioxidant system can handle HIV virus hiding inside the CD4 T cell of lymphocyte.
Fatemeh Mir Najafi Zadeh
University of New South Wales, Australia
Title: Synthesis of low toxic quantum dots for bioapplications
Time : 16:40-17:05
Biography:
Fatemeh Mir Najafi Zadeh has completed her PhD in 2015 from University of New South Wales, Australia. She is currently a Research Assistant in her previous research group in the Department of Chemistry at University of New South Wales. She has published several papers in peer-reviewed journals and has attended more than 14 international conferences as speaker or poster presenter.
Abstract:
The application of semiconductor nanoparticles, or quantum dots (QDs), in the biological context has received a great deal of attention over the last decade due to their unique optical properties, including narrow emission peaks which are tunable through the visible and near-infrared wavelengths, wide absorption bands, large molar extinction coefficients and high photo stabilities. However, there are some serious restrictions to bio applications of QDs such as toxicity, water solubility, biodegradation and photostability of the QDs in biological environments. This work reports cytotoxicity studies of CdSe(S), ZnSe(S) and CdSe(S)/ZnO QDs in the presence of two cell lines: human carcinoma cells (HCT-116) and human skin fibroblast cell line (WS1). The XRD results indicated that cubic phase nanocrystals were obtained in all synthesizes images revealed that all QDs were spherical nanoparticles and optical spectroscopy showed that they had narrow emission and wide absorption bands. The cytotoxicity data showed that cytotoxicity of QDs is dose-dependent and aqueous-synthesized QDs exhibit lower toxicity than organic-synthesized QDs. The results of cytotoxicity assays showed that the cells had 100% proliferation after 72h incubation with ZnSe(S) QDs, at all concentrations used in this study, whilst CdSe(S) QDs exhibit low toxicity at concentrations up to 105μg/ml. This indicates that cadmium is source of toxicity due to production of free radicals. Confocal microscopy showed that all cells nuclei were stable after incubation with QDs and there was no shift or change in emission wavelength of the obtained QDs in cell media, indicating that the obtained QDs are stable in biological environment.
Imran Tariq
Philipps University of Marburg, Germany