Pawandeep Kaur
Thapar Institute of Engineering and Technology, India
Title: Development and Characterization of Different Insulin Based Nanoformulations for Wound Healing Activity
Biography
Biography: Pawandeep Kaur
Abstract
Statement of the Problem: 422 million people, in 2016, have diabetes due to excess free body glucose in their body fluids which leads to various problems including retinopathy, neuropathy, arthritis, damage blood vessels and also causes a delay in wound healing. At the site of the wound, TNF-É‘, IFN-ϒ, IL-1β, and IL-6 pro-inflammatory cytokines causes generation of free radicals leading to inflammation which become persistent in diabetes. Insulin induces expression of IL-4/IL-13, IL-10 anti-inflammatory cytokines etc which further down regulates NFkβP65/P65 assembly. Insulin shifts the equilibrium towards NFkβP50/P65 and leads to down regulation of inflammatory cytokines like IL-6, IL-10 etc through STAT6, STAT3 and c-Maf activation causing inflammatory condition nullification. Insulin is a peptide hormone that plays multiple functions in our body, like control inflammation, increase in cell differentiation, lipid and protein biosynthesis, etc., and controlling glucose levels in the blood through glucose metabolism. The purpose of this study is to form the nanoformulations of insulin and use as a wound healing agent. Findings: The explored nanoformulations of insulin are biocompatible, stable, helps in cell division and migration, and wound healing. The IAgNP showed enhanced in vitro cell migration in both diabetic and no diabetic conditions compared to controls. In vivo histology evaluation showed a significant decrease in leukocyte infiltration, faster collagen deposition, and rapid re-epithelization anti-inflammatory activity, which is beneficial in treating chronic wounds. The in vivo studies showed a rapid decrease in inflammatory (IL-6 and TNF-α) cytokines and increased anti-inflammatory (IL-10) cytokines. IZnQCs and ICuQCs showed specific receptor binding, bioimaging, and wound healing properties. In IZnQCs, both insulin and zinc showed a synergistic effect through targeting IR and phosphorylating the same. Conclusion & Significance: These novel formulations are helpful in the wound recovery and wound monitoring wound and generate hope for better management of wounds, epically diabetic conditions.